Fluid and solid media for isolation of Brucella abortus
نویسندگان
چکیده
منابع مشابه
ISOLATION AND PURIFICATION OF MAJOR OUTER MEMBRANE PROTEINS FROM BRUCELLA ABORTUS S-99
Isolation and purification of major outer membrane proteins (OMP) from the cell wall envelope of Brucella abortus S-99 were achieved by sonication, solubilization and membrane fractionation in the presence of non-ionic detergent (Tx-100) and lysozyme treatments, followed by ultracentrifugation. The crude OMP was treated with trypsin to free the preparation from any other protein contaminan...
متن کاملPopulation Dynamics of Brucella on Solid Media.
An examination is presented of the growth characteristics on solid media of one strain each of Brucella abortus and B. neotomae. It was observed that, during the exponential growth phase, generation times of approximately 100 min were measured for both organisms under a variety of conditions. The logarithmic growth phase did not extend beyond 18 hr in most instances. It was noted that erythrito...
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Background: Classical bacteriological detection of Brucella species from food, and environment is routinely carried out based on morphological and biochemical characteristics. However, for increasing specificity and sensitivity of species identification methods, development of a molecular assay is necessary that was main aim of this study. Methods: Panel of some reference strains belonging to ...
متن کاملBrucella abortus endocarditis.
T HE purpose of this report is to describe the successful treatment of a case of bacterial endocarditis due to Brucella abortus. As far as I have been able to discover at the date of writing, no successfully treated case of this disease has been reported previously, with the exception of a case of mixed bacterial endocarditis presented in 1954 by Quinn and Brown.' A second case of treated Br. a...
متن کاملisolation of brucella abortus using pcr-rflp analysis
brucella transmission and epidemiology depend on infecting species and biovar. therefore, exact identification of the brucella is important to design correct control and treatment strategies. in this study, we examined presence of other brucellae in isfahan. one hundred twenty brucella isolates were collected and genomic dna was extracted from them. omp2a fragment of all isolates were amplified...
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ژورنال
عنوان ژورنال: Journal of Hygiene
سال: 1975
ISSN: 0022-1724
DOI: 10.1017/s0022172400046878